Splicing Factor 1 antibody (N-Term)

Catalog No. ABIN2777968

The Rabbit Polyclonal anti-Splicing Factor 1 antibody (ABIN2777968) specifically detects Splicing Factor 1 in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Zebrafish (Danio rerio), Guinea Pig, Goat and Saccharomyces cerevisiae samples.

Quick Overview for Splicing Factor 1 antibody (N-Term) (ABIN2777968)

Target: Splicing Factor 1 (SF1)

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Zebrafish (Danio rerio), Guinea Pig, Goat, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Splicing Factor 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Splicing Factor 1 Antibody

Binding Specificity: N-Term

Purpose: SF1 Antibody - N-terminal region

Sequence: LRTGDLGIPP NPEDRSPSPE PIYNSEGKRL NTREFRTRKK LEEERHNLIT

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 90%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 91%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SF1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SF1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP33492-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Splicing Factor 1

Target: Splicing Factor 1 (SF1)

Alternative Name: SF1

Background:

Background Information: SF1 contains 1 CCHC-type zinc finger and 1 KH domain. SF1 is Necessary for the ATP-dependent first step of spliceosome assembly. It binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. SF1 may act as transcription repressor.

Gene Name: Splicing factor 1

Alternative Symbols: BBP, MBBP, ZFM1, ZNF162, D11S636, ZCCHC25

Protein Name: Splicing factor 1

Molecular Weight: 68kDa

Gene ID: 7536

NCBI Accession: NP_004621

UniProt: Q15637

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location