BMI1 antibody (Middle Region)

Catalog No. ABIN2778059

This Rabbit Polyclonal antibody specifically detects BMI1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Dog, Cow, Horse, Guinea Pig and Zebrafish (Danio rerio).

Quick Overview for BMI1 antibody (Middle Region) (ABIN2778059)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Horse, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BMI1 Antibody

Binding Specificity: Middle Region

Sequence: MDIPNTFQID VMYEEEPLKD YYTLMDIAYI YTWRRNGPLP LKYRVRPTCK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 93%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against Bmi1. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 324 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: Bmi1

Background: Bmi1 is a component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Alias Symbols: AW546694, Bmi-1, Pcgf4
Protein Interaction Partner: Rnf2, Eed, Aurkb, Phc2, RING1, Gata6, Mph1, Cbfb, Runx1, Cdkn2a, HIST2H2AC, Slc11a1, Dnmt3b, Kdm2b, Phc1, Cbx2, Phc3, Dmap1, CBX8, SCMH1, CBX4, SMARCA5, HSPA4,
Protein Size: 324

Molecular Weight: 37 kDa

Gene ID: 12151

NCBI Accession: NM_007552, NP_031578

UniProt: P25916

Pathways: Cell Division Cycle, Autophagy