Pirin antibody (C-Term)

Catalog No. ABIN2778063

The Rabbit Polyclonal anti-Pirin antibody is suitable to detect Pirin in samples from Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for Pirin antibody (C-Term) (ABIN2778063)

Target: Pirin (PIR)

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Pirin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Pirin Antibody

Binding Specificity: C-Term

Purpose: PIR Antibody - C-terminal region

Sequence: EPHHTAVLGE GDSVQVENKD PKRSHFVLIA GEPLREPVIQ HGPFVMNTNE

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against PIR. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PIR

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8108280

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Pirin

Target: Pirin (PIR)

Alternative Name: PIR

Background:

Background Information: PIR is a member of the cupin superfamily. The protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication.This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described.

Gene Name: Pirin (iron-binding nuclear protein)

Protein Name: Pirin

Molecular Weight: 32kDa

Gene ID: 8544

NCBI Accession: NP_003653

UniProt: O00625