MED26 antibody (N-Term)

Catalog No. ABIN2778100

This Rabbit Polyclonal antibody specifically detects MED26 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Rabbit, Horse, Pig, Zebrafish (Danio rerio) and Guinea Pig.

Quick Overview for MED26 antibody (N-Term) (ABIN2778100)

Target: MED26 (Mediator Complex Subunit 26 (MED26))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Horse, Pig, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED26 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MED26 Antibody

Binding Specificity: N-Term

Purpose: MED26 Antibody - N-terminal region

Sequence: SSLEKYPITK EALEETRLGK LINDVRKKTK NEELAKRAKK LLRSWQKLIE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MED26. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MED26

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP33745-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MED26

Target: MED26 (Mediator Complex Subunit 26 (MED26))

Alternative Name: MED26

Background:

Targeted Subunit: 26

Background Information: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. MED26 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-261 AC024075.4 20290-20550 c 262-726 AF104253.1 1-465 727-3184 AK128435.1 1414-3871

Gene Name: Mediator complex subunit 26

Alternative Symbols: CRSP7, CRSP70

Protein Name: Mediator of RNA polymerase II transcription subunit 26

Molecular Weight: 65kDa

Gene ID: 9441

NCBI Accession: NP_004822

UniProt: O95402

Pathways: Regulation of Lipid Metabolism by PPARalpha