PARP1 antibody (C-Term)

Catalog No. ABIN2778108

Product Details anti-PARP1 Antibody

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Horse, Rabbit, Cow, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: ISRLPKGKHS VKGLGKTTPD PSANISLDGV DVPLGTGISS GVIDTSLLYN

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against PARP1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PARP1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1014 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP1 (PARP1 Products)

Background: PARP1 encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. PARP1 can be cleaved resulting in fragements of 29 and 85 kDa.
Alias Symbols: C12orf6, PARP1
Protein Interaction Partner: MDFI, TRIP13, DCAF11, RNF114, OTUD4, LYZ, GTPBP3, NUP98,
Protein Size: 1014

Molecular Weight: 113 kDa

Gene ID: 57097

NCBI Accession: NM_001618, NP_001609

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location