Tiparp antibody (N-Term)

Catalog No. ABIN2778114

There are 2+ publications for this product available. The Rabbit Polyclonal anti-Tiparp antibody is suitable to detect Tiparp in samples from Human, Mouse, Rat, Cow, Dog, Horse, Pig and Rabbit. It has been validated for WB.

Quick Overview for Tiparp antibody (N-Term) (ABIN2778114)

Target: Tiparp (TCDD-Inducible Poly(ADP-Ribose) Polymerase (Tiparp))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tiparp antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Tiparp Antibody

Binding Specificity: N-Term

Purpose: TIPARP Antibody - N-terminal region

Sequence: LKTCFKKKDQ KRLGTGTLRS LRPILNTLLE SGSLDGVFRS RNQSTDENSL

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 83%, Pig: 100%, Rabbit: 92%, Rat: 83%

Characteristics: This is a rabbit polyclonal antibody against TIPARP. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TIPARP

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP33763-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Tiparp antibody (ABIN2778114)

MacPherson, Tamblyn, Rajendra, Bralha, McPherson, Matthews: "2,3,7,8-Tetrachlorodibenzo-p-dioxin poly(ADP-ribose) polymerase (TiPARP, ARTD14) is a mono-ADP-ribosyltransferase and repressor of aryl hydrocarbon receptor transactivation." in: Nucleic acids research, Vol. 41, Issue 3, pp. 1604-21, (2013) (PubMed).

Mehrle, Rosenfelder, Schupp, del Val, Arlt, Hahne, Bechtel, Simpson, Hofmann, Hide, Glatting, Huber, Pepperkok, Poustka, Wiemann: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, (2005) (PubMed).

Target Details for Tiparp

Target: Tiparp (TCDD-Inducible Poly(ADP-Ribose) Polymerase (Tiparp))

Alternative Name: TIPARP

Background:

Background Information: TIPARP is a poly [ADP-ribose] polymerase using NAD+ as a substrate to transfer ADP-ribose onto glutamic acid residues of a protein acceptor, repeated rounds of ADP-ribosylation leads to the formation of poly(ADPribose) chains on the protein, thereby altering the function of the target protein. TIPARP may play a role in the adaptative response to chemical exposure (TCDD) and thereby mediates certain effects of the chemicals.

Gene Name: TCDD-inducible poly(ADP-ribose) polymerase

Alternative Symbols: PARP7, ARTD14, pART14

Protein Name: TCDD-inducible poly [ADP-ribose] polymerase

Molecular Weight: 76 kDa

Gene ID: 25976

NCBI Accession: NP_056323

UniProt: Q7Z3E1

Pathways: Platelet-derived growth Factor Receptor Signaling