KIF2A antibody (N-Term)

Catalog No. ABIN2778183

The Rabbit Polyclonal anti-KIF2A antibody is suitable to detect KIF2A in samples from Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig and Rabbit. It has been validated for IHC and WB.

Quick Overview for KIF2A antibody (N-Term) (ABIN2778183)

Target: KIF2A (Kinesin Heavy Chain Member 2A (KIF2A))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIF2A antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-KIF2A Antibody

Binding Specificity: N-Term

Purpose: KIF2A Antibody - N-terminal region

Sequence: IEPSPETPPP PASSAKVNKI VKNRRTVASI KNDPPSRDNR VVGSARARPS

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 79%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against KIF2A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human KIF2A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP33902-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for KIF2A

Target: KIF2A (Kinesin Heavy Chain Member 2A (KIF2A))

Alternative Name: KIF2A

Background:

Background Information: KIF2A belongs to the kinesin-like protein family, MCAK/KIF2 subfamily. It contains 1 kinesin-motor domain. KIF2A plus end-directed microtubule-dependent motor required for normal brain development. It may regulate microtubule dynamics during axonal growth. KIF2A is implicated in formation of bipolar mitotic spindles. It has microtubule depolymerization activity. Hela cells lacking KIF2A show asymmetric or monopolar mitotic spindles. Osteosarcoma cells (U2OS) lacking KIF2A or KIF2B show disorganised or monopolar mitotic spindles.Kinesins, such as KIF2, are microtubule-associated motor proteins. For background information on kinesins, see MIM 148760.[supplied by OMIM].

Gene Name: Kinesin heavy chain member 2A

Alternative Symbols: HK2, KIF2, CDCBM3

Protein Name: Kinesin-like protein KIF2A

Molecular Weight: 80kDa

Gene ID: 3796

NCBI Accession: NP_004511

UniProt: O00139

Pathways: Microtubule Dynamics, Ribonucleoprotein Complex Subunit Organization