LIG4 antibody (N-Term)

Catalog No. ABIN2778272

The Rabbit Polyclonal anti-LIG4 antibody has been validated for WB and IHC. It is suitable to detect LIG4 in samples from Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig and Rabbit.

Quick Overview for LIG4 antibody (N-Term) (ABIN2778272)

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-LIG4 Antibody

Binding Specificity: N-Term

Sequence: DGERMQMHKD GDVYKYFSRN GYNYTDQFGA SPTEGSLTPF IHNAFKADIQ

Predicted Reactivity: Cow: 92%, Dog: 93%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 92%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against LIG4. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human LIG4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 911 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for LIG4

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Alternative Name: LIG4

Background: LIG4 encodes a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome.
Protein Interaction Partner: XRCC4, UBC, AGO2, GZMK, ZSCAN18, PHF10, PNKP, TSFM, PRKDC, GUSBP3, NOMO3, FBLL1, PGP, ASPM, AMZ2P1, PPP1R18, COMMD1, ZNF428, AGAP4, WDR20, RPS19BP1, ZFAND2A, FIP1L1, CCDC176, VKORC1, COA7, SOWAHC, SEMA4G, ALMS1, WARS, UBE2L3, UBB, TPT1, TPI1, TP53BP1, TLE
Protein Size: 911

Molecular Weight: 104 kDa

Gene ID: 3981

NCBI Accession: NM_002312, NP_002303

UniProt: Q8IY66

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response