Afadin antibody (N-Term)

Catalog No. ABIN2778340

This anti-Afadin antibody is a Rabbit Polyclonal antibody detecting Afadin in WB. Suitable for Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Rabbit, Pig, Saccharomyces cerevisiae and Zebrafish (Danio rerio).

Quick Overview for Afadin antibody (N-Term) (ABIN2778340)

Target: Afadin (MLLT4)

Reactivity: Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Rabbit, Pig, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Afadin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Afadin Antibody

Binding Specificity: N-Term

Sequence: GVIQNFKRTL SKKEKKEKKK REKEALRQAS DKDDRPFQGE DVENSRLAAE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 85%, Rat: 100%, Yeast: 92%, Zebrafish: 91%

Characteristics: This is a rabbit polyclonal antibody against MLLT4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MLLT4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 674 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Afadin

Target: Afadin (MLLT4)

Alternative Name: MLLT4

Background: AF6 is a Ras target that regulates cell-cell adhesions downstream of Ras activation. It is fused with MLL in leukemias caused by t(6,11) translocations.
Alias Symbols: RP3-470B24.4, AF-6, AF6, AFADIN, FLJ34371, RP3-431P23.3
Protein Interaction Partner: UBC, LGR4, LATS2, YWHAQ, YWHAE, VCAM1, SMAD2, ITGA4, GRB2, FN1, SIRT7, HNRNPA1, ARRB2, YWHAG, YWHAB, KMT2A, MEN1, PVRL4, PICK1, RIT2, SORBS2, F11R, NRXN2, SSX2IP, RIT1, NRXN3, PVRL3, PVRL1, MRAS, SORBS1, EPHB6, EPHA7, JAG1, EPHB3, RALGDS, TJP1, EPHB2, PVR
Protein Size: 674

Molecular Weight: 74 kDa

Gene ID: 4301

NCBI Accession: NM_001040000

UniProt: P55196

Pathways: Cell-Cell Junction Organization