AFF3 antibody (Middle Region)

Catalog No. ABIN501256

This Rabbit Polyclonal antibody specifically detects AFF3 in WB. It exhibits reactivity toward Human, Mouse, Pig, Rabbit, Rat and Dog.It has been mentioned in 1 publication

Quick Overview for AFF3 antibody (Middle Region) (ABIN501256)

Target: AFF3 (AF4/FMR2 Family, Member 3 (AFF3))

Reactivity: Human, Mouse, Pig, Rabbit, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFF3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AFF3 Antibody

Binding Specificity: Middle Region

Purpose: AFF3 Antibody - middle region

Sequence: APATKDSESA PPSHTSDTPA EKALPKSKRK RKCDNEDDYR EIKKSQGEKD

Predicted Reactivity: Dog: 86%, Human: 100%, Mouse: 93%, Pig: 86%, Rabbit: 77%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against AFF3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human AFF3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP34237-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-AFF3 antibody (ABIN501256)

To, Faseruk, Gokgoz, Pinnaduwage, Done, Andrulis: "LAF-4 is aberrantly expressed in human breast cancer." in: International journal of cancer. Journal international du cancer, Vol. 115, Issue 4, pp. 568-74, (2005) (PubMed).

Target Details for AFF3

Target: AFF3 (AF4/FMR2 Family, Member 3 (AFF3))

Alternative Name: AFF3

Background:

Background Information: AFF3 is a tissue-restricted nuclear transcriptional activator that is preferentially expressed in lymphoid tissue. Isolation of this protein initially defined a highly conserved LAF4/MLLT2 gene family of nuclear transcription factors that may function in lymphoid development and oncogenesis. In some ALL patients, this gene has been found fused to the gene for MLL.This gene encodes a tissue-restricted nuclear transcriptional activator that is preferentially expressed in lymphoid tissue. Isolation of this protein initially defined a highly conserved LAF4/MLLT2 gene family of nuclear transcription factors that may function in lymphoid development and oncogenesis. In some ALL patients, this gene has been found fused to the gene for MLL. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.

Gene Name: AF4/FMR2 family, member 3

Alternative Symbols: LAF4, MLLT2-like

Protein Name: AF4/FMR2 family member 3

Molecular Weight: 134 kDa

Gene ID: 3899

NCBI Accession: NP_002276

UniProt: P51826