Aprataxin antibody (C-Term)

Catalog No. ABIN2778569

This Rabbit Polyclonal antibody specifically detects Aprataxin in IHC and WB. It exhibits reactivity toward Human, Mouse, Rat, Guinea Pig, Dog, Horse, Rabbit and Cowand has been mentioned in 1 publication.

Quick Overview for Aprataxin antibody (C-Term) (ABIN2778569)

Target: Aprataxin (APTX)

Reactivity: Human, Mouse, Rat, Guinea Pig, Dog, Horse, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aprataxin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-Aprataxin Antibody

Binding Specificity: C-Term

Sequence: VIEMVQEAGR VTVRDGMPEL LKLPLRCHEC QQLLPSIPQL KEHLRKHWTQ

Predicted Reactivity: Cow: 100%, Dog: 92%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against APTX. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human APTX

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 342 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Aprataxin antibody (ABIN2778569)

Dopeso, Mateo-Lozano, Elez, Landolfi, Ramos Pascual, Hernández-Losa, Mazzolini, Rodrigues, Bazzocco, Carreras, Espín, Armengol, Wilson, Mariadason, Ramon Y Cajal, Tabernero, Schwartz, Arango: "Aprataxin tumor levels predict response of colorectal cancer patients to irinotecan-based treatment." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 16, Issue 8, pp. 2375-82, (2010) (PubMed).

Target Details for Aprataxin

Target: Aprataxin (APTX)

Alternative Name: APTX

Background: APTX is a member of the histidine triad (HIT) superfamily, some of which have nucleotide-binding and diadenosine polyphosphate hydrolase activities. APTX may play a role in single-stranded DNA repair. Mutations in this gene have been associated with ataxia-ocular apraxia.This gene encodes a member of the histidine triad (HIT) superfamily, some of which have nucleotide-binding and diadenosine polyphosphate hydrolase activities. The encoded protein may play a role in single-stranded DNA repair. Mutations in this gene have been associated with ataxia-ocular apraxia. Multiple transcript variants encoding distinct isoforms have been identified for this gene, however, the full length nature of some variants has not been determined.This gene encodes a member of the histidine triad (HIT) superfamily, some of which have nucleotide-binding and diadenosine polyphosphate hydrolase activities. The encoded protein may play a role in single-stranded DNA repair. Mutations in this gene have been associated with ataxia-ocular apraxia. Multiple transcript variants encoding distinct isoforms have been identified for this gene, however, the full length nature of some variants has not been determined.
Alias Symbols: AOA, AOA1, AXA1, EAOH, EOAHA, FHA-HIT
Protein Interaction Partner: PNMA1, UBC, SUMO1, NEDD8, LIG3, HSPA4, XRCC1, DDX21, CNTROB, TSPYL2, CALCOCO1, SYT17, ZNF639, MAPKBP1, CEP350, PICK1, XRCC4, TRIM37, MBP, HIVEP1, BANF1, PARP1, TP53,
Protein Size: 342

Molecular Weight: 38 kDa

Gene ID: 54840

NCBI Accession: NM_175073, NP_778243

UniProt: Q6JV85

Pathways: DNA Damage Repair, Chromatin Binding