RNF8 antibody (C-Term)

Catalog No. ABIN2778597

The Rabbit Polyclonal anti-RNF8 antibody is suitable to detect RNF8 in samples from Human, Mouse, Rat, Cow, Horse, Pig, Dog and Rabbit. It has been validated for WB and IHC.

Quick Overview for RNF8 antibody (C-Term) (ABIN2778597)

Target: RNF8 (Ring Finger Protein 8 (RNF8))

Reactivity: Human, Mouse, Rat, Cow, Horse, Pig, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RNF8 Antibody

Binding Specificity: C-Term

Sequence: MEELNRSKKD FEAIIQAKNK ELEQTKEEKE KMQAQKEEVL SHMNDVLENE

Predicted Reactivity: Cow: 100%, Dog: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 92%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against RNF8. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RNF8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 485 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RNF8

Target: RNF8 (Ring Finger Protein 8 (RNF8))

Alternative Name: RNF8

Background: RNF8 contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins.The protein encoded by this gene contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Alias Symbols: FLJ12013, KIAA0646
Protein Interaction Partner: TMEM79, HOMEZ, SEPT3, RBFOX2, PNMA2, USP16, WASL, HERC2, MAD1L1, VCP, TNFRSF1A, TNF, BCL10, UBE2N, UBC, NEDD8, JMJD1C, MDC1, EXOSC2, MDM2, BLM, SEPT8, SEPT7, RPSA, H2AFX, RL2, HIST2H2BE, HIST2H2AC, PCNA, RAD50, NBN, MRE11A, UBE2E3, UBE2V1, UBE2E2, UBE2E1,
Protein Size: 485

Molecular Weight: 55 kDa

Gene ID: 9025

NCBI Accession: NM_003958, NP_003949

UniProt: O76064

Pathways: Production of Molecular Mediator of Immune Response