RNF34 antibody (Middle Region)

Catalog No. ABIN2778633

This Rabbit Polyclonal antibody specifically detects RNF34 in WB. It exhibits reactivity toward Human, Rat, Cow, Dog, Rabbit, Horse and Guinea Pig.

Quick Overview for RNF34 antibody (Middle Region) (ABIN2778633)

Target: RNF34 (Ring Finger Protein 34 (RNF34))

Reactivity: Human, Rat, Cow, Dog, Rabbit, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF34 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RNF34 Antibody

Binding Specificity: Middle Region

Purpose: RNF34 Antibody - middle region

Sequence: LVDLVLCHHG LGSEDDMDTS SLNSSRSQTS SFFTRSFFSN YTAPSATMSS

Predicted Reactivity: Cow: 87%, Dog: 93%, Guinea Pig: 80%, Horse: 93%, Human: 100%, Rabbit: 80%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against RNF34. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RNF34

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40158-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RNF34

Target: RNF34 (Ring Finger Protein 34 (RNF34))

Alternative Name: RNF34

Background:

Background Information: RNF34 contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis.The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Gene Name: Ring finger protein 34, E3 ubiquitin protein ligase

Alternative Symbols: RFI, RIF, RIFF, hRFI, CARP1, CARP-1

Protein Name: E3 ubiquitin-protein ligase RNF34

Molecular Weight: 42kDa

Gene ID: 80196

NCBI Accession: NP_079402

UniProt: Q969K3