Cytokeratin 18 antibody (N-Term)

Catalog No. ABIN2778669

The Rabbit Polyclonal anti-Cytokeratin 18 antibody has been validated for WB and IHC. It is suitable to detect Cytokeratin 18 in samples from Human, Mouse, Rat, Dog, Cow, Horse, Rabbit and Guinea Pig.

Quick Overview for Cytokeratin 18 antibody (N-Term) (ABIN2778669)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Cytokeratin 18 Antibody

Binding Specificity: N-Term

Sequence: TRSTFSTNYR SLGSVQAPSY GARPVSSAAS VYAGAGGSGS RISVSRSTSF

Predicted Reactivity: Cow: 86%, Dog: 92%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against KRT18. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human KRT18

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 430 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: KRT18

Background: KRT18 (Keratin 18) is type I intermediate filament chain. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis.KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
Alias Symbols: K18, CYK18
Protein Interaction Partner: KRT18, GOLGA2, UBC, HAUS1, CCDC146, MDM2, EED, HNRNPU, FLII, FKBP3, DDX1, C14orf166, RTCB, CNOT1, SEC16A, RPL14, RFC2, UPF2, BBS7, PAN2, BBS4, BBS2, BBS1, SMARCD1, ORC5, NOS2, KRT8, KPNA2, ITGA4, HRAS, GRN, FN1, EP300, ATF2, CDH1, CDC5L, YTHDC1, PIAS4, TX
Protein Size: 430

Molecular Weight: 47 kDa

Gene ID: 3875

NCBI Accession: NM_000224, NP_000215

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis