U2AF2 antibody (C-Term)

Catalog No. ABIN2778723

The Rabbit Polyclonal anti-U2AF2 antibody (ABIN2778723) specifically detects U2AF2 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Horse, Dog, Guinea Pig, Zebrafish (Danio rerio) and Saccharomyces cerevisiae samples.

Quick Overview for U2AF2 antibody (C-Term) (ABIN2778723)

Target: U2AF2 (U2AF59) (U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59))

Reactivity: Human, Mouse, Rat, Cow, Horse, Dog, Guinea Pig, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This U2AF2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-U2AF2 Antibody

Binding Specificity: C-Term

Purpose: U2AF2 Antibody - C-terminal region

Sequence: TEVLCLMNMV LPEELLDDEE YEEIVEDVRD ECSKYGLVKS IEIPRPVDGV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Yeast: 79%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against U2AF2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human U2AF2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40285-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for U2AF2

Target: U2AF2 (U2AF59) (U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59))

Alternative Name: U2AF2

Background:

Background Information: U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2AF2 is the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly.

Gene Name: U2 small nuclear RNA auxiliary factor 2

Alternative Symbols: U2AF65

Protein Name: U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2, isoform CRA_a EMBL EDL31286.1

Molecular Weight: 53kDa

Gene ID: 11338

NCBI Accession: NP_001012496

UniProt: Q80XR5