CARS antibody (Middle Region)

Catalog No. ABIN2778727

The Rabbit Polyclonal anti-CARS antibody (ABIN2778727) specifically detects CARS in WB. The antibody is reactive with Human, Mouse, Rat, Rabbit, Dog, Horse, Cow, Zebrafish (Danio rerio) and Guinea Pig samples.

Quick Overview for CARS antibody (Middle Region) (ABIN2778727)

Target: CARS (Cysteinyl-tRNA Synthetase (CARS))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Horse, Cow, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CARS antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CARS Antibody

Binding Specificity: Middle Region

Purpose: CARS Antibody - middle region

Sequence: QEQEAAKLAK MKIPPSEMFL SETDKYSKFD ENGLPTHDME GKELSKGQAK

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 86%, Rabbit: 79%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against CARS. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CARS

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40294-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CARS

Target: CARS (Cysteinyl-tRNA Synthetase (CARS))

Alternative Name: CARS

Background:

Background Information: CARS is a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. CARS gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Gene Name: Cysteinyl-tRNA synthetase

Alternative Symbols: CARS, MDBH, CYSRS, MCDDBH, MGC:11246

Protein Name: Cysteine--tRNA ligase, cytoplasmic

Molecular Weight: 85 kDa

Gene ID: 833

NCBI Accession: NP_001014437

UniProt: Q5HYE4