CARS antibody (C-Term)

Catalog No. ABIN2778728

Cited in 1 publication. The Rabbit Polyclonal anti-CARS antibody (ABIN2778728) specifically detects CARS in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Rabbit, Dog, Horse, Cow, Zebrafish (Danio rerio), Pig and Saccharomyces cerevisiae samples.

Quick Overview for CARS antibody (C-Term) (ABIN2778728)

Target: CARS (Cysteinyl-tRNA Synthetase (CARS))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Horse, Cow, Zebrafish (Danio rerio), Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CARS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CARS Antibody

Binding Specificity: C-Term

Purpose: CARS Antibody - C-terminal region

Sequence: KRKKKEEAAR RKQEQEAAKL AKMKIPPSEM FLSETDKYSK FDENGLPTHD

Predicted Reactivity: Cow: 79%, Dog: 93%, Horse: 93%, Human: 100%, Mouse: 92%, Pig: 85%, Rabbit: 100%, Rat: 100%, Yeast: 91%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against CARS. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CARS

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8098285

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-CARS antibody (ABIN2778728)

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).

Target Details for CARS

Target: CARS (Cysteinyl-tRNA Synthetase (CARS))

Alternative Name: CARS

Background:

Background Information: CARS is a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Gene Name: Cysteinyl-tRNA synthetase

Alternative Symbols: CARS, MDBH, CYSRS, MCDDBH, MGC:11246

Molecular Weight: 81kDa

Gene ID: 833

NCBI Accession: NP_001014438

UniProt: P49589