RED1 antibody (N-Term)

Catalog No. ABIN2778757

Cited in 1 publication. The Rabbit Polyclonal anti-RED1 antibody (ABIN2778757) specifically detects RED1 in WB. The antibody is reactive with Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Dog, Horse, Guinea Pig and Rabbit samples.

Quick Overview for RED1 antibody (N-Term) (ABIN2778757)

Target: RED1 (ADARB1) (Adenosine Deaminase, RNA-Specific, B1 (ADARB1))

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Dog, Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RED1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RED1 Antibody

Binding Specificity: N-Term

Purpose: ADARB1 Antibody - N-terminal region

Sequence: QLSNGGGGGP GRKRPLEEGS NGHSKYRLKK RRKTPGPVLP KNALMQLNEI

Predicted Reactivity: Cow: 83%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against ADARB1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ADARB1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8094116

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-RED1 antibody (ABIN2778757)

Spellman, Ahmed, Dubach, Gardiner: "Expression of trisomic proteins in Down syndrome model systems." in: Gene, Vol. 512, Issue 2, pp. 219-25, (2012) (PubMed).

Target Details for RED1

Target: RED1 (ADARB1) (Adenosine Deaminase, RNA-Specific, B1 (ADARB1))

Alternative Name: ADARB1

Background:

Background Information: ADARB1 is an enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site.This gene encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region.

Gene Name: Adenosine deaminase, RNA-specific, B1

Alternative Symbols: RED1, ADAR2, DRABA2, DRADA2, NEDHYMS

Protein Name: Double-stranded RNA-specific editase 1

Molecular Weight: 77kDa

Gene ID: 104

NCBI Accession: NP_001103

UniProt: Q4AE79