DAZL antibody (C-Term)

Catalog No. ABIN2778769

The Rabbit Polyclonal anti-DAZL antibody is suitable to detect DAZL in samples from Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse and Sheep. It has been validated for WB.

Quick Overview for DAZL antibody (C-Term) (ABIN2778769)

Target: DAZL (Deleted in Azoospermia-Like (DAZL))

Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAZL antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DAZL Antibody

Binding Specificity: C-Term

Purpose: DAZL Antibody - C-terminal region

Sequence: EVDPGAEVVP NECSVHEATP PSGNGPQKKS VDRSIQTVVS CLFNPENRLR

Predicted Reactivity: Cow: 93%, Dog: 93%, Goat: 93%, Guinea Pig: 86%, Horse: 92%, Human: 100%, Mouse: 93%, Rat: 86%, Sheep: 93%

Characteristics: This is a rabbit polyclonal antibody against DAZL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human DAZL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40356-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for DAZL

Target: DAZL (Deleted in Azoospermia-Like (DAZL))

Alternative Name: DAZL

Background:

Background Information: DAZ (Deleted in AZoospermia) is the potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. DAZL is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of the autosomal gene encoding DAZL during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in the Dazl gene have been linked to severe spermatogenic failure and infertility in males.The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Deleted in azoospermia-like

Alternative Symbols: DAZH, DAZL1, DAZLA, SPGYLA

Protein Name: Deleted in azoospermia-like

Molecular Weight: 33kDa

Gene ID: 1618

NCBI Accession: NP_001342

UniProt: Q92904