EIF4B antibody (Middle Region)

Catalog No. ABIN2778772

The Rabbit Polyclonal anti-EIF4B antibody (ABIN2778772) specifically detects EIF4B in WB. The antibody is reactive with Human, Mouse, Rat, Horse, Cow, Guinea Pig, Rabbit and Dog samples.

Quick Overview for EIF4B antibody (Middle Region) (ABIN2778772)

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Reactivity: Human, Mouse, Rat, Horse, Cow, Guinea Pig, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF4B Antibody

Binding Specificity: Middle Region

Purpose: EIF4B Antibody - middle region

Sequence: QTGNSSRGPG DGGNRDHWKE SDRKDGKKDQ DSRSAPEPKK PEENPASKFS

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 92%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against EIF4B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EIF4B

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40360-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF4B

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Alternative Name: EIF4B

Background:

Background Information: IF4B contains 1 RRM (RNA recognition motif) domain and is required for the binding of mRNA to ribosomes. Functions of EIF4B are in close association with EEF4-F and EIF4-A. It binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP and promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Gene Name: Eukaryotic translation initiation factor 4B

Alternative Symbols: EIF-4B, PRO1843

Protein Name: Eukaryotic translation initiation factor 4B

Molecular Weight: 69kDa

Gene ID: 1975

NCBI Accession: NP_001408

UniProt: P23588