ISG20 antibody (N-Term)

Catalog No. ABIN2778795

Product Details anti-ISG20 Antibody

Target: ISG20 (Interferon Stimulated Exonuclease Gene 20kDa (ISG20))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse, Dog, Horse, Cow, Pig, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ISG20 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GAVLYDKFIR PEGEITDYRT RVSGVTPQHM VGATPFAVAR LEILQLLKGK

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 79%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against ISG20. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ISG20

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 181 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ISG20 antibody (ABIN2778795)

Zahoor, Xue, Sato, Murakami, Takeshima, Aida: "HIV-1 Vpr induces interferon-stimulated genes in human monocyte-derived macrophages." in: PLoS ONE, Vol. 9, Issue 8, pp. e106418, (2014) (PubMed).

Horio, Murai, Inoue, Hamasaki, Tanaka, Ohgi: "Crystal structure of human ISG20, an interferon-induced antiviral ribonuclease." in: FEBS letters, Vol. 577, Issue 1-2, pp. 111-6, (2004) (PubMed).

Target Details for ISG20

Target: ISG20 (Interferon Stimulated Exonuclease Gene 20kDa (ISG20))

Alternative Name: ISG20 (ISG20 Products)

Synonyms: ISG20 antibody, CD25 antibody, HEM45 antibody, 1600023I01Rik antibody, 2010107M23Rik antibody, 20kDa antibody, DnaQL antibody, interferon stimulated exonuclease gene 20 antibody, interferon-stimulated protein antibody, ISG20 antibody, Isg20 antibody

Background: ISG20 belongs to the the exonuclease superfamily. It is exonuclease with specificity for single-stranded RNA and, to a lesser extent for DNA. It degrades RNA at a rate that is approximately 35-fold higher than its rate for single-stranded DNA. It is involved in the antiviral function of IFN against RNA viruses.
Alias Symbols: CD25, HEM45
Protein Interaction Partner: UBC,
Protein Size: 181

Molecular Weight: 20 kDa

Gene ID: 3669

NCBI Accession: NM_002201, NP_002192

UniProt: Q96AZ6