PIGF antibody (N-Term)

Catalog No. ABIN2778804

This Rabbit Polyclonal antibody specifically detects PIGF in IHC and WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Pig and Sheep.It has been mentioned in 1 publication

Quick Overview for PIGF antibody (N-Term) (ABIN2778804)

Target: PIGF (Phosphatidylinositol Glycan F (PIGF))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGF antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-PIGF Antibody

Binding Specificity: N-Term

Purpose: PIGF Antibody - N-terminal region

Sequence: MKDNDIKRLL YTHLLCIFSI ILSVFIPSLF LENFSILETH LTWLCICSGF

Predicted Reactivity: Cow: 86%, Dog: 93%, Horse: 92%, Human: 100%, Mouse: 86%, Pig: 86%, Rat: 86%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against PIGF. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PIGF

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8108025

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-PIGF antibody (ABIN2778804)

Mohammed, Nasreen, Tepper, Antony: "Cyclic stretch induces PlGF expression in bronchial airway epithelial cells via nitric oxide release." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 292, Issue 2, pp. L559-66, (2007) (PubMed).

Target Details for PIGF

Target: PIGF (Phosphatidylinositol Glycan F (PIGF))

Alternative Name: PIGF

Background:

Background Information: PIGF is a protein involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor, a glycolipid containing three mannose molecules in its core backbone, is found on many blood cells where it serves to anchor proteins to the cell surface. PIGF and another GPI synthesis protein, PIGO, function in the transfer of ethanolaminephosphate to the third mannose in GPI. This gene encodes a protein involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor, a glycolipid containing three mannose molecules in its core backbone, is found on many blood cells where it serves to anchor proteins to the cell surface. The encoded protein and another GPI synthesis protein, PIGO, function in the transfer of ethanolaminephosphate to the third mannose in GPI. Alternatively spliced transcript variants encoding different isoforms have been described.

Gene Name: Phosphatidylinositol glycan anchor biosynthesis, class F

Alternative Symbols: MGC32646, MGC33136

Protein Name: Phosphatidylinositol-glycan biosynthesis class F protein

Molecular Weight: 25kDa

Gene ID: 5281

NCBI Accession: NP_002634

UniProt: Q07326

Pathways: Inositol Metabolic Process