PPP1R8 antibody (Middle Region)

Catalog No. ABIN2778806

The Rabbit Polyclonal anti-PPP1R8 antibody (ABIN2778806) specifically detects PPP1R8 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig and Zebrafish (Danio rerio) samples.

Quick Overview for PPP1R8 antibody (Middle Region) (ABIN2778806)

Target: PPP1R8 (Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PPP1R8 Antibody

Binding Specificity: Middle Region

Purpose: PPP1R8 Antibody - middle region

Sequence: PNLAPDVDLT PVVPSAVNMN PAPNPAVYNP EAVNEPKKKK YAKEAWPGKK

Specificity: This antibody recognizes isoform-gamma

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 91%

Characteristics: This is a rabbit polyclonal antibody against PPP1R8. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PPP1R8

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8109399

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PPP1R8

Target: PPP1R8 (Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8))

Alternative Name: PPP1R8

Background:

Background Information: This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.

Gene Name: Protein phosphatase 1, regulatory subunit 8

Alternative Symbols: ARD1, ARD-1, NIPP1, NIPP-1, PRO2047

Protein Name: Nuclear inhibitor of protein phosphatase 1

Molecular Weight: 14kDa

Gene ID: 5511

NCBI Accession: NP_002704

UniProt: Q12972