PSMA1 antibody (C-Term)

Catalog No. ABIN2778810

Cited in 1 publication. The Rabbit Polyclonal anti-PSMA1 antibody (ABIN2778810) specifically detects PSMA1 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit and Cow samples.

Quick Overview for PSMA1 antibody (C-Term) (ABIN2778810)

Target: PSMA1 (Proteasome Subunit alpha Type 1 (PSMA1))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PSMA1 Antibody

Binding Specificity: C-Term

Sequence: TYLERHMSEF MECNLNELVK HGLRALRETL PAEQDLTTKN VSIGIVGKDL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against PSMA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PSMA1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 263 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PSMA1 antibody (ABIN2778810)

Cron, Zhu, Kushwaha, Hsieh, Merzon, Rameseder, Chen, DAndrea, Kozono: "Proteasome inhibitors block DNA repair and radiosensitize non-small cell lung cancer." in: PLoS ONE, Vol. 8, Issue 9, pp. e73710, (2013) (PubMed).

Target Details for PSMA1

Target: PSMA1 (Proteasome Subunit alpha Type 1 (PSMA1))

Alternative Name: PSMA1

Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA1 is a member of the peptidase T1A family which is a 20S core alpha subunit.The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified.The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified.
Alias Symbols: HC2, MGC14542, MGC14575, MGC14751, MGC1667, MGC21459, MGC22853, MGC23915, NU, PROS30
Protein Interaction Partner: PRDM14, CEP70, CCDC102B, GNPTAB, CEP72, KCTD9, ROPN1, ABI3, SH3GLB1, APIP, LDOC1, MTUS2, MAPRE3, MAPRE1, IKZF3, MID2, EHMT2, PNMA2, IKZF1, TRIM10, HUWE1, HOMER3, PNMA1, KRT38, BLZF1, MAD1L1, MKRN3, REL, PSMA3, MLH1, KRT31, KRT15, KRTAP5-9, GOLGA2, CDA, CC
Protein Size: 263

Molecular Weight: 29 kDa

Gene ID: 5682

NCBI Accession: NM_002786, NP_002777

UniProt: P25786

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA