RPN1 antibody (Middle Region)

Catalog No. ABIN2778827

The Rabbit Polyclonal anti-RPN1 antibody has been validated for WB. It is suitable to detect RPN1 in samples from Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig and Zebrafish (Danio rerio). There is 1 publication available.

Quick Overview for RPN1 antibody (Middle Region) (ABIN2778827)

Target: RPN1 (Ribophorin 1 (RPN1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPN1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RPN1 Antibody

Binding Specificity: Middle Region

Sequence: AAEARMKVAC ITEQVLTLVN KRIGLYRHFD ETVNRYKQSR DISTLNSGKK

Predicted Reactivity: Cow: 93%, Dog: 86%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against RPN1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RPN1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 607 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-RPN1 antibody (ABIN2778827)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for RPN1

Target: RPN1 (Ribophorin 1 (RPN1))

Alternative Name: RPN1

Background: This protein is a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: DKFZp686B16177, OST1, RBPH1
Protein Interaction Partner: UBQLN1, UBC, SGTA, SUMO3, STAU1, LGR4, SUMO1, NEDD8, MDM2, ASB5, ASB11, BMI1, EED, RNF2, EGFR, ADRB2, CLN5, CLN3, AMFR, OST4, MMS19, env, UBL4A, WHSC1, VCAM1, FN1, ATF2, VCP, ESR1, ECT2, STT3B, SND1, SYNCRIP, TECR, VDAC2, SSR3, RPN2, RPSA, ILF3, SLC25A5,
Protein Size: 607

Molecular Weight: 66 kDa

Gene ID: 6184

NCBI Accession: NM_002950, NP_002941

UniProt: P04843