YARS antibody (C-Term)

Catalog No. ABIN2778855

The Rabbit Polyclonal anti-YARS antibody is suitable to detect YARS in samples from Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio), Goat and Pig. It has been validated for WB.

Quick Overview for YARS antibody (C-Term) (ABIN2778855)

Target: YARS (Yars) (Tyrosyl-tRNA Synthetase (Yars))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio), Goat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YARS antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-YARS Antibody

Binding Specificity: C-Term

Purpose: YARS Antibody - C-terminal region

Sequence: QVEPLDPPAG SAPGEHVFVK GYEKGQPDEE LKPKKKVFEK LQADFKISEE

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 91%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against YARS. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human YARS

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8115095

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for YARS

Target: YARS (Yars) (Tyrosyl-tRNA Synthetase (Yars))

Alternative Name: YARS

Background:

Background Information: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.

Gene Name: Tyrosyl-tRNA synthetase

Alternative Symbols: YRS, YTS, YARS, TYRRS, CMTDIC

Protein Name: Tyrosine--tRNA ligase, cytoplasmic

Molecular Weight: 58kDa

Gene ID: 8565

NCBI Accession: NP_003671

UniProt: P54577