EIF3G antibody (N-Term)

Catalog No. ABIN2778866

The Rabbit Polyclonal anti-EIF3G antibody is suitable to detect EIF3G in samples from Human, Mouse, Rat, Dog, Cow, Pig, Guinea Pig and Zebrafish (Danio rerio). It has been validated for WB and IHC.

Quick Overview for EIF3G antibody (N-Term) (ABIN2778866)

Target: EIF3G (Eukaryotic Translation Initiation Factor 3, Subunit G (EIF3G))

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3G antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EIF3G Antibody

Binding Specificity: N-Term

Purpose: EIF3S4 Antibody - N-terminal region

Sequence: SPEPELLPGA PLPPPKEVIN GNIKTVTEYK IDEDGKKFKI VRTFRIETRK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against EIF3S4. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EIF3S4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8099958

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF3G

Target: EIF3G (Eukaryotic Translation Initiation Factor 3, Subunit G (EIF3G))

Alternative Name: EIF3S4

Background:

Targeted Subunit: G

Background Information: EIF3S4 contains 1 RRM (RNA recognition motif) domain. It binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. This subunit binds to the 18S rRNA.

Gene Name: Eukaryotic translation initiation factor 3, subunit G

Alternative Symbols: EIF3S4, EIF3-P42, eIF3-p44, eIF3-delta

Protein Name: Eukaryotic translation initiation factor 3 subunit G

Molecular Weight: 35kDa

Gene ID: 8666

NCBI Accession: NP_003746

UniProt: O75821

Pathways: Ribonucleoprotein Complex Subunit Organization