IREB2 antibody (Middle Region)

Catalog No. ABIN2778884

This Rabbit Polyclonal antibody specifically detects IREB2 in WB. It exhibits reactivity toward Human, Rat, Mouse, Pig, Dog, Horse, Guinea Pig, Rabbit and Zebrafish (Danio rerio).

Quick Overview for IREB2 antibody (Middle Region) (ABIN2778884)

Target: IREB2 (Iron-Responsive Element Binding Protein 2 (IREB2))

Reactivity: Human, Rat, Mouse, Pig, Dog, Horse, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IREB2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IREB2 Antibody

Binding Specificity: Middle Region

Sequence: IQINLNSIVP SVSGPKRPQD RVAVTDMKSD FQACLNEKVG FKGFQIAAEK

Predicted Reactivity: Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against IREB2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human IREB2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 963 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for IREB2

Target: IREB2 (Iron-Responsive Element Binding Protein 2 (IREB2))

Alternative Name: IREB2

Background: IREB2 binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. IREB2 binds to the IRE element in ferritin which results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.
Alias Symbols: ACO3, FLJ23381, IRP2, IRP2AD
Protein Interaction Partner: RBCK1, UBC, FAM96A, FAM96B, CIAO1, FBXL5, UBA2, TNPO1, CBS, TERT, USP53, APP, PSMA3, VHL, YBX1,
Protein Size: 963

Molecular Weight: 105 kDa

Gene ID: 3658

NCBI Accession: NM_004136, NP_004127

UniProt: P48200

Pathways: Transition Metal Ion Homeostasis