EIF4E2 antibody (N-Term)

Catalog No. ABIN2778915

Cited in 1 publication. The Rabbit Polyclonal anti-EIF4E2 antibody (ABIN2778915) specifically detects EIF4E2 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse and Rabbit samples.

Quick Overview for EIF4E2 antibody (N-Term) (ABIN2778915)

Target: EIF4E2 (Eukaryotic Translation Initiation Factor 4E Family Member 2 (EIF4E2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4E2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EIF4E2 Antibody

Binding Specificity: N-Term

Sequence: KDDDSGDHDQ NEENSTQKDG EKEKTERDKN QSSSKRKAVV PGPAEHPLQY

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 86%, Rabbit: 79%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against EIF4E2. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EIF4E2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 245 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EIF4E2 antibody (ABIN2778915)

Tee, Tee, Blenis: "Characterizing the interaction of the mammalian eIF4E-related protein 4EHP with 4E-BP1." in: FEBS letters, Vol. 564, Issue 1-2, pp. 58-62, (2004) (PubMed).

Target Details for EIF4E2

Target: EIF4E2 (Eukaryotic Translation Initiation Factor 4E Family Member 2 (EIF4E2))

Alternative Name: EIF4E2

Background: EIF4E2 belongs to the eukaryotic initiation factor 4E family. It recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Alias Symbols: 4EHP, IF4e, 4E-LP, EIF4EL3
Protein Interaction Partner: USHBP1, CARD9, PRDM14, HOMEZ, TRIM54, NECAB2, ADAMTSL4, AMOTL2, SPAG5, SPRY2, HUWE1, TMCC2, MAGED1, TCF4, TADA2A, TRIM27, REL, MYOG, KRT31, KRT19, KRT13, CDR2, AES, MAPRE3, NOTCH2NL, KRTAP10-8, KRTAP10-5, KRTAP10-9, ZBTB9, SPERT, USP54, MIPOL1, KRT40, UBX
Protein Size: 245

Molecular Weight: 27 kDa

Gene ID: 9470

NCBI Accession: NM_004846, NP_004837

UniProt: O60573

Pathways: SARS-CoV-2 Protein Interactome