HNRNPF antibody (C-Term)

Catalog No. ABIN2778920

This Rabbit Polyclonal antibody specifically detects HNRNPF in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Dog and Horseand has been mentioned in 1 publication.

Quick Overview for HNRNPF antibody (C-Term) (ABIN2778920)

Target: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNRNPF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HNRNPF Antibody

Binding Specificity: C-Term

Sequence: LNSTTGASNG AYSSQVMQGM GVSAAQATYS GLESQSVSGC YGAGYSGQNS

Predicted Reactivity: Cow: 91%, Dog: 92%, Guinea Pig: 100%, Horse: 92%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against HNRPF. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HNRPF

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 415 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HNRNPF antibody (ABIN2778920)

White, Gonsior, Bauer, Krämer-Albers, Luhmann, Trotter: "Heterogeneous nuclear ribonucleoprotein (hnRNP) F is a novel component of oligodendroglial RNA transport granules contributing to regulation of myelin basic protein (MBP) synthesis." in: The Journal of biological chemistry, Vol. 287, Issue 3, pp. 1742-54, (2012) (PubMed).

Target Details for HNRNPF

Target: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Alternative Name: HNRPF

Background: HNRPF belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH.This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH.
Alias Symbols: HNRPF, mcs94-1, OK/SW-cl.23
Protein Interaction Partner: HSFY1, C1orf94, CCDC33, EIF4ENIF1, RBFOX2, IKZF3, HNRNPUL1, HNRNPM, FUS, TFG, DZIP3, UBC, AES, NEDD1, CEP250, SUMO2, SUMO3, TP53, CDC20, STAU1, IVNS1ABP, SUMO1, NEDD8, MDM2, WWOX, RPA3, RPA2, RPA1, ERG, EED, rev, PRPF40A, OSGEP, ABCF3, DNAAF5, PPME1, PPP1
Protein Size: 415

Molecular Weight: 46 kDa

Gene ID: 3185

NCBI Accession: NM_004966, NP_004957

UniProt: P52597