PCBP2 antibody (N-Term)

Catalog No. ABIN2778922

This anti-PCBP2 antibody is a Rabbit Polyclonal antibody detecting PCBP2 in WB. Suitable for Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Dog and Rabbit.

Quick Overview for PCBP2 antibody (N-Term) (ABIN2778922)

Target: PCBP2 (Poly(rC) Binding Protein 2 (PCBP2))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCBP2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PCBP2 Antibody

Binding Specificity: N-Term

Sequence: KKGESVKKMR EESGARINIS EGNCPERIIT LAGPTNAIFK AFAMIIDKLE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against PCBP2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PCBP2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 366 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PCBP2

Target: PCBP2 (Poly(rC) Binding Protein 2 (PCBP2))

Alternative Name: PCBP2

Background: PCBP2 appears to be multifunctional. It along with PCBP-1 and hnRNPK corresponds to the major cellular poly(rC)-binding proteins. This protein together with PCBP-1 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This gene and PCBP-1 has paralogues PCBP3 and PCBP4 which is thought to arose as a result of duplication events of entire genes.The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: HNRPE2, MGC110998, hnRNP-E2
Protein Interaction Partner: FUS, HUWE1, UBC, SNRPA, SUMO2, SUMO3, CEP250, MDM2, EED, TARDBP, ADRB2, IGSF8, HMGA1, CD81, HMGA2, WDR83, SLU7, CASK, UBL4A, VCAM1, PCBP2, NOS2, ITGA4, FN1, BRCA1, PRPF40A, SF3B2, NKAP, WDR77, PAXIP1, BARD1, PPIB, EEF1A1, FBXO6, UBD, PCBP1, QKI, CDK2, COP
Protein Size: 366

Molecular Weight: 39 kDa

Gene ID: 5094

NCBI Accession: NM_005016, NP_005007

UniProt: Q6IPF4