Nucleolin antibody (N-Term)

Catalog No. ABIN2778935

The Rabbit Polyclonal anti-Nucleolin antibody has been validated for WB and IHC. It is suitable to detect Nucleolin in samples from Human, Mouse, Rat, Cow, Pig, Rabbit, Dog, Horse, Saccharomyces cerevisiae and Zebrafish (Danio rerio). There is 1 publication available.

Quick Overview for Nucleolin antibody (N-Term) (ABIN2778935)

Target: Nucleolin (NCL)

Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Dog, Horse, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Nucleolin Antibody

Binding Specificity: N-Term

Sequence: GKALVATPGK KGAAIPAKGA KNGKNAKKED SDEEEDDDSE EDEEDDEDED

Predicted Reactivity: Cow: 87%, Dog: 93%, Horse: 93%, Human: 100%, Mouse: 91%, Pig: 86%, Rabbit: 77%, Rat: 93%, Yeast: 83%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against NCL. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NCL

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 710 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Nucleolin antibody (ABIN2778935)

Shefer, Boulos, Gotea, Arafat, Ben Chaim, Muharram, Isaac, Eden, Sperling, Elnitski, Sperling: "A novel role for nucleolin in splice site selection." in: RNA biology, Vol. 19, Issue 1, pp. 333-352, (2022) (PubMed).

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: NCL

Background: Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus.Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
Alias Symbols: C23
Protein Interaction Partner: HUWE1, PIK3R1, PA2G4, NCL, HNRNPD, AURKA, MDM2, NFE4, CDKN1A, ADAP1, STAU1, ADAP2, UBC, NUP62, ZBTB1, ARFGEF1, RPA3, RPA2, RPA1, ERG, RNF2, SUZ12, EED, EZH2, BMI1, NPM3, EIF3G, XRCC5, PLOD2, NCBP1, MCM2, LMNA, PRMT1, HNRNPK, KCTD12, DNM1, ACLY, SRPK3, TAR
Protein Size: 710

Molecular Weight: 78 kDa

Gene ID: 4691

NCBI Accession: NM_005381, NP_005372

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization