PIGK antibody (N-Term)

Catalog No. ABIN2778939

The Rabbit Polyclonal anti-PIGK antibody is suitable to detect PIGK in samples from Human, Mouse, Rat, Guinea Pig, Rabbit, Cow, Horse, Zebrafish (Danio rerio) and Dog. It has been validated for WB.

Quick Overview for PIGK antibody (N-Term) (ABIN2778939)

Target: PIGK (GPI8) (GPI-anchor transamidase (GPI8))

Reactivity: Human, Mouse, Rat, Guinea Pig, Rabbit, Cow, Horse, Zebrafish (Danio rerio), Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PIGK Antibody

Binding Specificity: N-Term

Purpose: PIGK Antibody - N-terminal region

Sequence: SVYRSVKRLG IPDSHIVLML ADDMACNPRN PKPATVFSHK NMELNVYGDD

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 93%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against PIGK. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PIGK

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8101874

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PIGK

Target: PIGK (GPI8) (GPI-anchor transamidase (GPI8))

Alternative Name: PIGK

Background:

Background Information: PIGK is a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. PIGK is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. This gene encodes a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Phosphatidylinositol glycan anchor biosynthesis, class K

Alternative Symbols: GPI8, NEDHCAS

Protein Name: GPI-anchor transamidase

Molecular Weight: 43kDa

Gene ID: 10026

NCBI Accession: NP_005473

UniProt: Q92643