EMG1 antibody (N-Term)

Catalog No. ABIN2778974

There is 1 publication for this product available. The Rabbit Polyclonal anti-EMG1 antibody is suitable to detect EMG1 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow and Zebrafish (Danio rerio). It has been validated for WB and IHC.

Quick Overview for EMG1 antibody (N-Term) (ABIN2778974)

Target: EMG1 (EMG1 Nucleolar Protein Homolog (EMG1))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EMG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EMG1 Antibody

Binding Specificity: N-Term

Purpose: EMG1 Antibody - N-terminal region

Sequence: SLETVKVGKT YELLNCDKHK SILLKNGRDP GEARPDITHQ SLLMLMDSPL

Predicted Reactivity: Cow: 92%, Dog: 92%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 93%, Rabbit: 92%, Rat: 93%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against EMG1. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EMG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8099581

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-EMG1 antibody (ABIN2778974)

Armistead, Khatkar, Meyer, Mark, Patel, Coghlan, Lamont, Liu, Wiechert, Cattini, Koetter, Wrogemann, Greenberg, Entian, Zelinski, Triggs-Raine: "Mutation of a gene essential for ribosome biogenesis, EMG1, causes Bowen-Conradi syndrome." in: American journal of human genetics, Vol. 84, Issue 6, pp. 728-39, (2009) (PubMed).

Target Details for EMG1

Target: EMG1 (EMG1 Nucleolar Protein Homolog (EMG1))

Alternative Name: EMG1

Background:

Background Information: EMG1 involved in 40S ribosomal subunit biogenesis. It seems to play a role a methylation reaction in pre-rRNA processing.

Gene Name: EMG1 nucleolar protein homolog (S. cerevisiae)

Alternative Symbols: C2F, NEP1, Grcc2f

Protein Name: Ribosomal RNA small subunit methyltransferase NEP1

Molecular Weight: 27kDa

Gene ID: 10436

NCBI Accession: NP_006322

UniProt: Q92979