NXF1 antibody (N-Term)

Catalog No. ABIN2778977

This anti-NXF1 antibody is a Rabbit Polyclonal antibody detecting NXF1 in WB and IHC. Suitable for Human, Mouse, Rat, Dog, Guinea Pig and Cow.

Quick Overview for NXF1 antibody (N-Term) (ABIN2778977)

Target: NXF1 (Nuclear RNA Export Factor 1 (NXF1))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NXF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NXF1 Antibody

Binding Specificity: N-Term

Sequence: MADEGKSYSE HDDERVNFPQ RKKKGRGPFR WKYGEGNRRS GRGGSGIRSS

Predicted Reactivity: Cow: 92%, Dog: 83%, Guinea Pig: 92%, Human: 100%, Mouse: 92%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against NXF1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NXF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 619 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NXF1

Target: NXF1 (Nuclear RNA Export Factor 1 (NXF1))

Alternative Name: NXF1

Background: NXF1 is one member of a family of nuclear RNA export factor. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. NXF1 shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses.This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses.
Alias Symbols: TAP, MEX67
Protein Interaction Partner: KRT40, TIFA, GFAP, LDOC1, NUP62, MID2, TP53BP2, DVL3, SUMO2, UBC, SUZ12, EED, BMI1, TARDBP, NXT1, NUP98, RANBP2, ESRRA, KCTD5, TRA2A, SGTA, TRA2B, SRSF1, RAD51, ZBED2, CSNK2A1, EIF2A, ILF3, HNRNPC, HNRNPK, DHX15, DHX9, DDB1, DDX19A, LUC7L3, HP1BP3, RBMX,
Protein Size: 619

Molecular Weight: 68 kDa

Gene ID: 10482

NCBI Accession: NM_006362, NP_006353

UniProt: Q9UBU9