PRPF8 antibody (N-Term)

Catalog No. ABIN2778986

The Rabbit Polyclonal anti-PRPF8 antibody is suitable to detect PRPF8 in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse and Rabbit. It has been validated for WB.

Quick Overview for PRPF8 antibody (N-Term) (ABIN2778986)

Target: PRPF8 (PRP8 Pre-mRNA Processing Factor 8 (PRPF8))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRPF8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PRPF8 Antibody

Binding Specificity: N-Term

Purpose: PRPF8 Antibody - N-terminal region

Sequence: SHRHSVKSQE PLPDDDEEFE LPEFVEPFLK DTPLYTDNTA NGIALLWAPR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against PRPF8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PRPF8

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40649-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PRPF8

Target: PRPF8 (PRP8 Pre-mRNA Processing Factor 8 (PRPF8))

Alternative Name: PRPF8

Background:

Background Information: Pre-mRNA splicing occurs in 2 sequential transesterification steps. PRPF8 is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. The gene encoding PRPF8 is a candidate gene for autosomal dominant retinitis pigmentosa.Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae)

Alternative Symbols: PRP8, RP13, HPRP8, PRPC8, SNRNP220

Protein Name: Pre-mRNA-processing-splicing factor 8

Molecular Weight: 273kDa

Gene ID: 10594

NCBI Accession: NP_006436

UniProt: Q6P2Q9