SF3A3 antibody (Middle Region)

Catalog No. ABIN2779010

This Rabbit Polyclonal antibody specifically detects SF3A3 in WB. It exhibits reactivity toward Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Horse, Rabbit, Guinea Pig and Saccharomyces cerevisiae.It has been mentioned in 1 publication

Quick Overview for SF3A3 antibody (Middle Region) (ABIN2779010)

Target: SF3A3 (Splicing Factor 3a, Subunit 3, 60kDa (SF3A3))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Horse, Rabbit, Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SF3A3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SF3A3 Antibody

Binding Specificity: Middle Region

Sequence: THENVQRKQA RTGEEREEEE EEQISESESE DEENEIIYNP KNLPLGWDGK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 91%

Characteristics: This is a rabbit polyclonal antibody against SF3A3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SF3A3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 501 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SF3A3 antibody (ABIN2779010)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for SF3A3

Target: SF3A3 (Splicing Factor 3a, Subunit 3, 60kDa (SF3A3))

Alternative Name: SF3A3

Background: SF3A3 is the subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP.This This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: PRP9, PRPF9, SAP61, SF3a60
Protein Interaction Partner: TRIM69, HUWE1, ZNF212, TADA2A, FUS, UBC, NEDD8, MDM2, WWOX, RNF2, SUZ12, EED, rev, APBB1, ACTN1, ACTN4, STAM2, HGS, SNRPB, CDAN1, PRPF40A, WBP4, TCERG1, UBD, SF3A1, FN1, gag-pol, PRMT5, PRPF19, PRPF31, SF3B1, SF3B3, SF3B4, HNRNPR, DDX46, TOX4, DDX23, PRPF
Protein Size: 501

Molecular Weight: 59 kDa

Gene ID: 10946

NCBI Accession: NM_006802, NP_006793

UniProt: Q12874

Pathways: Ribonucleoprotein Complex Subunit Organization