CPSF1 antibody (Middle Region)

Catalog No. ABIN2779041

The Rabbit Polyclonal anti-CPSF1 antibody is suitable to detect CPSF1 in samples from Human, Rat and Horse. It has been validated for WB.

Quick Overview for CPSF1 antibody (Middle Region) (ABIN2779041)

Target: CPSF1 (Cleavage and Polyadenylation Specific Factor 1, 160kDa (CPSF1))

Reactivity: Human, Rat, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPSF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CPSF1 Antibody

Binding Specificity: Middle Region

Purpose: CPSF1 Antibody - middle region

Sequence: GCYDMWTVIA PVRKEEEDNP KGEGTEQEPS TTPEADDDGR RHGFLILSRE

Predicted Reactivity: Horse: 79%, Human: 100%, Rat: 77%

Characteristics: This is a rabbit polyclonal antibody against CPSF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CPSF1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40741-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CPSF1

Target: CPSF1 (Cleavage and Polyadenylation Specific Factor 1, 160kDa (CPSF1))

Alternative Name: CPSF1

Background:

Targeted Subunit: 1

Background Information: CPSF1 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.

Gene Name: Cleavage and polyadenylation specific factor 1, 160 kDa

Alternative Symbols: MYP27, CPSF160, P/cl.18, HSU37012

Protein Name: Cleavage and polyadenylation specificity factor subunit 1

Molecular Weight: 161kDa

Gene ID: 29894

NCBI Accession: NP_037423

UniProt: Q10570