NIP7 antibody (C-Term)

Catalog No. ABIN2779096

Cited in 1 publication. The Rabbit Polyclonal anti-NIP7 antibody (ABIN2779096) specifically detects NIP7 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Pig, Horse, Rabbit, Zebrafish (Danio rerio), Guinea Pig and Saccharomyces cerevisiae samples.

Quick Overview for NIP7 antibody (C-Term) (ABIN2779096)

Target: NIP7 (Nuclear Import 7 Homolog (NIP7))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Horse, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NIP7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NIP7 Antibody

Binding Specificity: C-Term

Purpose: NIP7 Antibody - C-terminal region

Sequence: VVVYSMADIP LGFGVAAKST QDCRKVDPMA IVVFHQADIG EYVRHEETLT

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 93%, Yeast: 79%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against NIP7. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NIP7

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8106746

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-NIP7 antibody (ABIN2779096)

Zinzalla, Stracka, Oppliger, Hall: "Activation of mTORC2 by association with the ribosome." in: Cell, Vol. 144, Issue 5, pp. 757-68, (2011) (PubMed).

Target Details for NIP7

Target: NIP7 (Nuclear Import 7 Homolog (NIP7))

Alternative Name: NIP7

Background:

Targeted Subunit: biogenesis protein NIP7 homolog

Background Information: NIP7 contains 1PUA domain and belongs to the NIP7 family. It may play a role in 60S ribosomal subunit synthesis.

Gene Name: Nuclear import 7 homolog (S. cerevisiae)

Alternative Symbols: KD93, CGI-37, HSPC031

Protein Name: 60S ribosome subunit biogenesis protein NIP7 homolog

Molecular Weight: 20kDa

Gene ID: 51388

NCBI Accession: NP_057185

UniProt: Q9Y221

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly