CPSF2 antibody (N-Term)

Catalog No. ABIN2779108

Product Details anti-CPSF2 Antibody

Target: CPSF2 (Cleavage and Polyadenylation Specific Factor 2, 100kDa (CPSF2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPSF2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: YAVGKLGLNC AIYATIPVYK MGQMFMYDLY QSRHNTEDFT LFTLDDVDAA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against CPSF2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CPSF2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 782 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CPSF2 antibody (ABIN2779108)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for CPSF2

Target: CPSF2 (Cleavage and Polyadenylation Specific Factor 2, 100kDa (CPSF2))

Alternative Name: CPSF2 (CPSF2 Products)

Synonyms: CPSF100 antibody, 100kDa antibody, 2610024B04Rik antibody, AI662483 antibody, Cpsf antibody, MCPSF antibody, mKIAA1367 antibody, zgc:92484 antibody, cpsf2-A antibody, cleavage and polyadenylation specific factor 2 antibody, cleavage and polyadenylation specific factor 2 S homeolog antibody, cleavage and polyadenylation specificity factor subunit 2 antibody, CPSF2 antibody, Cpsf2 antibody, cpsf2 antibody, PAAG_07931 antibody, cpsf2.S antibody, LOC582050 antibody, LOC100157277 antibody, LOC100179344 antibody

Background: CPSF2 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. It belongs to the metallo-beta-lactamase superfamily, RNA-metabolizing metallo-beta-lactamase-like family, CPSF2/YSH1 subfamily.
Alias Symbols: CPSF100, KIAA1367
Protein Interaction Partner: RBBP6, UBC, WWOX, EZH2, rev, HECW2, CLK2, NPM1, CPSF3, SYMPK, CSTF2, MDC1, ECT2, BARD1, GTF3C6, FIP1L1, WDR33, POLE3, CPSF1, RALBP1, MED17, GTF3C3, SMARCD3, SMARCA4, POLR2A, CUL3, HNRNPA1, ELAVL1, SUMO2, SMARCAD1, CSTF2T, RRAGA, TBP, PSMA3, CLP1, CDC73, R
Protein Size: 782

Molecular Weight: 88 kDa

Gene ID: 53981

NCBI Accession: NM_017437, NP_059133

UniProt: Q9P2I0