CPSF2 antibody (N-Term)

Catalog No. ABIN2779108

This Rabbit Polyclonal antibody specifically detects CPSF2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit and Zebrafish (Danio rerio).It has been mentioned in 1 publication

Quick Overview for CPSF2 antibody (N-Term) (ABIN2779108)

Target: CPSF2 (Cleavage and Polyadenylation Specific Factor 2, 100kDa (CPSF2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPSF2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CPSF2 Antibody

Binding Specificity: N-Term

Purpose: CPSF2 Antibody - N-terminal region

Sequence: YAVGKLGLNC AIYATIPVYK MGQMFMYDLY QSRHNTEDFT LFTLDDVDAA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against CPSF2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CPSF2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8097523

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-CPSF2 antibody (ABIN2779108)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for CPSF2

Target: CPSF2 (Cleavage and Polyadenylation Specific Factor 2, 100kDa (CPSF2))

Alternative Name: CPSF2

Background:

Targeted Subunit: 2

Background Information: CPSF2 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. It belongs to the metallo-beta-lactamase superfamily, RNA-metabolizing metallo-beta-lactamase-like family, CPSF2/YSH1 subfamily.

Gene Name: Cleavage and polyadenylation specific factor 2, 100 kDa

Alternative Symbols: CPSF100

Protein Name: Cleavage and polyadenylation specificity factor subunit 2

Molecular Weight: 88kDa

Gene ID: 53981

NCBI Accession: NP_059133

UniProt: Q9P2I0