NXF3 antibody (C-Term)

Catalog No. ABIN2779117

This anti-NXF3 antibody is a Rabbit Polyclonal antibody detecting NXF3 in WB and IHC. Suitable for Human, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit and Rat.

Quick Overview for NXF3 antibody (C-Term) (ABIN2779117)

Target: NXF3 (Nuclear RNA Export Factor 3 (NXF3))

Reactivity: Human, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NXF3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NXF3 Antibody

Binding Specificity: C-Term

Sequence: SSFLVDMWYQ TEWMLCFSVN GVFKEVEGQS QGSVLAFTRT FIATPGSSSS

Predicted Reactivity: Cow: 92%, Dog: 92%, Guinea Pig: 77%, Horse: 79%, Human: 100%, Pig: 92%, Rabbit: 92%, Rat: 77%

Characteristics: This is a rabbit polyclonal antibody against NXF3. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NXF3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 531 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NXF3

Target: NXF3 (Nuclear RNA Export Factor 3 (NXF3))

Alternative Name: NXF3

Background: NXF3 is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. NXF3 has shortened LRR and ubiquitin-associated domains and its RDB is unable to bind RNA. It is located in the nucleoplasm but is not associated with either the nuclear envelope or the nucleolus.This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene has shortened LRR and ubiquitin-associated domains and its RDB is unable to bind RNA. It is located in the nucleoplasm but is not associated with either the nuclear envelope or the nucleolus.
Protein Interaction Partner: NXT2, NXT1, SKIL, NXF1, HHV8GK18_gp81, HNRNPUL1, XPO1, NUP214,
Protein Size: 531

Molecular Weight: 58 kDa

Gene ID: 56000

NCBI Accession: NM_022052, NP_071335

UniProt: Q9H4D5