NXF3 antibody (AA 50-130)

Catalog No. ABIN7223602

The Rabbit Polyclonal anti-NXF3 antibody (ABIN7223602) specifically detects NXF3 in ELISA, WB, IHC and IF. The antibody is reactive with Human samples.

Quick Overview for NXF3 antibody (AA 50-130) (ABIN7223602)

Target: NXF3 (Nuclear RNA Export Factor 3 (NXF3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NXF3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-NXF3 Antibody

Binding Specificity: AA 50-130

Purpose: Rabbit Anti-NXF3 Polyclonal Antibody

Specificity: NXF3 Polyclonal Antibody detects endogenous levels of NXF3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human NXF3 at AA range: 50-130

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NXF3

Target: NXF3 (Nuclear RNA Export Factor 3 (NXF3))

Alternative Name: NXF3

Background: NXF3, TAPL3, Nuclear RNA export factor 3, TAP-like protein 3, TAPL-3NXF3 (nuclear RNA export factor 3) is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of NXF3 has shortened LRR and ubiquitin-associated domains and its RDB is unable to bind RNA. It is located in the nucleoplasm but is not associated with either the nuclear envelope or the nucleolus.

Molecular Weight: 60kD

Gene ID: 56000

UniProt: Q9H4D5