RBM28 antibody (C-Term)

Catalog No. ABIN2779126

This Rabbit Polyclonal antibody specifically detects RBM28 in WB and IHC. It exhibits reactivity toward Human, Mouse, Dog, Guinea Pig, Horse, Rat, Rabbit, Cow and Zebrafish (Danio rerio) and has been mentioned in 2+ publications.

Quick Overview for RBM28 antibody (C-Term) (ABIN2779126)

Target: RBM28 (RNA Binding Motif Protein 28 (RBM28))

Reactivity: Human, Mouse, Dog, Guinea Pig, Horse, Rat, Rabbit, Cow, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBM28 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RBM28 Antibody

Binding Specificity: C-Term

Sequence: QTKAEVEQVE LPDGKKRRKV LALPSHRGPK IRLRDKGKVK PVHPKKPKPQ

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against RBM28. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RBM28

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 759 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-RBM28 antibody (ABIN2779126)

Nousbeck, Spiegel, Ishida-Yamamoto, Indelman, Shani-Adir, Adir, Lipkin, Bercovici, Geiger, van Steensel, Steijlen, Bergman, Bindereif, Choder, Shalev, Sprecher: "Alopecia, neurological defects, and endocrinopathy syndrome caused by decreased expression of RBM28, a nucleolar protein associated with ribosome biogenesis." in: American journal of human genetics, Vol. 82, Issue 5, pp. 1114-21, (2008) (PubMed).

Suzuki, Yoshitomo-Nakagawa, Maruyama, Suyama, Sugano: "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library." in: Gene, Vol. 200, Issue 1-2, pp. 149-56, (1997) (PubMed).

Target Details for RBM28

Target: RBM28 (RNA Binding Motif Protein 28 (RBM28))

Alternative Name: RBM28

Background: RBM28 protein may be a specific nucleolar component of the spliceosomal snRNPs.
Protein Interaction Partner: SUMO3, UBC, TARBP2, LIN28A, EED, RNF2, HECW2, HDAC11, ESR1, ZNF622, RRP12, POLR3G, POLR3F, RPS17, RPL5, DKC1, CTPS1, CAND1, CUL1, CUL3, SIRT7, SUMO2, SUMO1, NOP56,
Protein Size: 759

Molecular Weight: 83 kDa

Gene ID: 55131

NCBI Accession: NM_018077, NP_060547

UniProt: Q9NW13

Pathways: SARS-CoV-2 Protein Interactome