SKP2 antibody (C-Term)

Catalog No. ABIN2779304

The Rabbit Polyclonal anti-SKP2 antibody is suitable to detect SKP2 in samples from Human, Rat, Cow, Pig, Horse, Dog, Guinea Pig and Rabbit. It has been validated for WB.

Quick Overview for SKP2 antibody (C-Term) (ABIN2779304)

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Reactivity: Human, Rat, Cow, Pig, Horse, Dog, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKP2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SKP2 Antibody

Binding Specificity: C-Term

Sequence: TLQLLKEALP HLQINCSHFT TIARPTIGNK KNQEIWGIKC RLTLQKPSCL

Predicted Reactivity: Cow: 100%, Dog: 92%, Guinea Pig: 83%, Horse: 92%, Human: 100%, Pig: 100%, Rabbit: 100%, Rat: 75%

Characteristics: This is a rabbit polyclonal antibody against SKP2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human SKP2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SKP2

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Alternative Name: SKP2

Background: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class, in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas.
Protein Interaction Partner: USP18, CUL1, CDKN1A, SAMHD1, SUGT1, UBC, AR, RND3, CKS1B, MYC, SMAD7, CDKN1B, SKP1, CDK3, CDK2, LATS2, FZR1, CEP192, SF3B3, KMT2A, E2F1, KPNA6, SIRT3, SIRT4, RBX1, CDC14B, NEDD8, SIRT6, KPNA1, HSP90AA1, EP300, CEBPA, CDH1, BRCA1, BARD1, BTRC, SNRPB2, CDC3
Protein Size: 210

Molecular Weight: 23 kDa

Gene ID: 6502

Pathways: Mitotic G1-G1/S Phases