LMX1A antibody (Middle Region)

Catalog No. ABIN2779408

The Rabbit Polyclonal anti-LMX1A antibody (ABIN2779408) specifically detects LMX1A in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig and Rabbit samples.

Quick Overview for LMX1A antibody (Middle Region) (ABIN2779408)

Target: LMX1A (LIM Homeobox Transcription Factor 1, alpha (LMX1A))

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LMX1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-LMX1A Antibody

Binding Specificity: Middle Region

Purpose: LMX1A Antibody - middle region

Sequence: QNQRAKMKKL ARRQQQQQQD QQNTQRLSSA QTNGGGSAGM EGIMNPYTAL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 86%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against LMX1A. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human LMX1A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8104457

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for LMX1A

Target: LMX1A (LIM Homeobox Transcription Factor 1, alpha (LMX1A))

Alternative Name: LMX1A

Background:

Background Information: Insulin is produced exclusively by the beta cells in the islets of Langerhans in the pancreas. The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear genes that bind to specific sequences within the promoter of the insulin gene (INS, MIM 176730) and interact with RNA polymerase to activate or repress transcription. LMX1 is a homeodomain protein that binds an A/T-rich sequence in the insulin promoter and stimulates transcription of insulin. Insulin is produced exclusively by the beta cells in the islets of Langerhans in the pancreas. The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear genes that bind to specific sequences within the promoter of the insulin gene (INS, MIM 176730) and interact with RNA polymerase to activate or repress transcription. LMX1 is a homeodomain protein that binds an A/T-rich sequence in the insulin promoter and stimulates transcription of insulin (German et al., 1994 [PubMed 7698771]).[supplied by OMIM]. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-199 AL160058.8 14249-14447 c 200-2447 AK127724.1 362-2609 2448-3382 AL390730.12 9415-10349 c

Gene Name: LIM homeobox transcription factor 1, alpha

Alternative Symbols: LMX1, DFNA7, LMX1.1

Protein Name: LIM homeobox transcription factor 1-alpha

Molecular Weight: 43kDa

Gene ID: 4009

NCBI Accession: NP_796372

UniProt: Q8TE12

Pathways: Dopaminergic Neurogenesis