SMAD7 antibody (N-Term)

Catalog No. ABIN2779418

Product Details anti-SMAD7 Antibody

Target: SMAD7 (SMAD, Mothers Against DPP Homolog 7 (SMAD7))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD7 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LVRRLWRSRA PGGEDEEEGA GGGGGGGELR GEGATDSRAH GAGGGGPGRA

Predicted Reactivity: Cow: 93%, Dog: 93%, Human: 100%, Mouse: 92%, Pig: 93%, Rabbit: 93%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against SMAD7. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SMAD7

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 426 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SMAD7 antibody (ABIN2779418)

Briones-Orta, Sosa-Garrocho, Moreno-Alvarez, Fonseca-Sánchez, Macías-Silva: "SnoN co-repressor binds and represses smad7 gene promoter." in: Biochemical and biophysical research communications, Vol. 341, Issue 3, pp. 889-94, (2006) (PubMed).

Target Details for SMAD7

Target: SMAD7 (SMAD, Mothers Against DPP Homolog 7 (SMAD7))

Alternative Name: SMAD7 (SMAD7 Products)

Background: Smad7 acts to functionally inactivate RB and de-repress E2F without blocking the activation of TbetaRI and the nuclear translocation of Smad2/3, allowing TGF-beta1 to exert effects in a cancer cell that is resistant to TGF-beta1-mediated growth inhibition.
Alias Symbols: FLJ16482, MADH7, MADH8, CRCS3
Protein Interaction Partner: HDAC3, HDAC2, USP11, SMURF2, SMURF1, SKP2, MYC, Melk, HCVgp1, RNF111, KDM2A, SOX13, PIAS1, SOX7, UBE2O, MKL2, ASH1L, PIAS4, CXXC5, ZNF107, ZBTB11, HEYL, TTF2, UBC, TTF1, SOX5, MYOD1, XRCC6, CTNNB1, MAPK14, BAMBI, PPP1R15A, NEDD4L, STAMBP, YAP1, AXIN1, TP5
Protein Size: 426

Molecular Weight: 46 kDa

Gene ID: 4092

NCBI Accession: NM_005904, NP_005895

UniProt: O15105

Pathways: Interferon-gamma Pathway, Cell-Cell Junction Organization