SMAD7 antibody (AA 1-100)

Catalog No. ABIN670206

Product Details anti-SMAD7 Antibody

Target: SMAD7 (SMAD, Mothers Against DPP Homolog 7 (SMAD7))

Binding Specificity: AA 1-100

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Smad7

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SMAD7 antibody (ABIN670206)

Yang, Ding, Xu, Chen, Ding: "MicroRNA-15a inhibition protects against hypoxia/reoxygenation-induced apoptosis of cardiomyocytes by targeting mothers against decapentaplegic homolog 7." in: Molecular medicine reports, Vol. 15, Issue 6, pp. 3699-3705, (2017) (PubMed).

Liu, Shi, Yang, Zhao, Du, Zou, Yu: "Synchronous alteration pattern between serine-threonine kinase receptor-associated protein and Smad7 in pilocarpine-induced rats of epilepsy." in: Synapse (New York, N.Y.), Vol. 68, Issue 6, pp. 275-82, (2014) (PubMed).

Kou, Hu, Yao, Wang, Shen, Kang, Hong: "Transforming growth factor-?1 promotes Treg commitment in nasal polyposis after intranasal steroid treatment." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 62, Issue 3, pp. 283-9, (2013) (PubMed).

Target Details for SMAD7

Target: SMAD7 (SMAD, Mothers Against DPP Homolog 7 (SMAD7))

Alternative Name: Smad7 (SMAD7 Products)

Synonyms: CRCS3 antibody, MADH7 antibody, MADH8 antibody, Smad7a antibody, Madh7 antibody, Smad10 antibody, madh7 antibody, xsmad7 antibody, wu:fj60c10 antibody, SMAD family member 7 antibody, SMAD family member 7 L homeolog antibody, SMAD7 antibody, Smad7 antibody, smad7.L antibody, smad7 antibody

Background:

Synonyms: CRCS3, MADH7, MADH8, Mothers against decapentaplegic homolog 7, MAD homolog 7, Mothers against DPP homolog 7, Mothers against decapentaplegic homolog 8, MAD homolog 8, Mothers against DPP homolog 8, SMAD family member 7, SMAD 7, Smad7, hSMAD7

Background: Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members, has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity).

Molecular Weight: 46kDa

Gene ID: 4092

UniProt: O15105

Pathways: Interferon-gamma Pathway, Cell-Cell Junction Organization