ASCL2 antibody (Middle Region)

Catalog No. ABIN2779425

This Rabbit Polyclonal antibody specifically detects ASCL2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit and Zebrafish (Danio rerio).It has been mentioned in 1 publication

Quick Overview for ASCL2 antibody (Middle Region) (ABIN2779425)

Target: ASCL2 (Achaete-Scute Complex Homolog 2 (ASCL2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASCL2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ASCL2 Antibody

Binding Specificity: Middle Region

Purpose: ASCL2 Antibody - middle region

Sequence: QALRQHVPHG GASKKLSKVE TLRSAVEYIR ALQRLLAEHD AVRNALAGGL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 92%, Rabbit: 77%, Rat: 100%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against ASCL2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ASCL2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP32025-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ASCL2 antibody (ABIN2779425)

Schwitalla, Fingerle, Cammareri, Nebelsiek, Göktuna, Ziegler, Canli, Heijmans, Huels, Moreaux, Rupec, Gerhard, Schmid, Barker, Clevers, Lang, Neumann, Kirchner, Taketo, van den Brink, Sansom, Arkan et al.: "Intestinal tumorigenesis initiated by dedifferentiation and acquisition of stem-cell-like properties. ..." in: Cell, Vol. 152, Issue 1-2, pp. 25-38, (2013) (PubMed).

Target Details for ASCL2

Target: ASCL2 (Achaete-Scute Complex Homolog 2 (ASCL2))

Alternative Name: ASCL2

Background:

Background Information: AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system.This gene is a member of the basic helix-loop-helix (BHLH) family of transcription factors. It activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. Involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1649 BC028140.1 1-1649 1650-1864 BC057801.1 1286-1500

Gene Name: Achaete-scute complex homolog 2 (Drosophila)

Alternative Symbols: ASH2, HASH2, MASH2, bHLHa45

Protein Name: Achaete-scute homolog 2

Molecular Weight: 20 kDa

Gene ID: 430

NCBI Accession: NP_005161

UniProt: Q99929

Pathways: Stem Cell Maintenance