IRX2 antibody (Middle Region)

Catalog No. ABIN2779496

There is 1 publication for this product available. The Rabbit Polyclonal anti-IRX2 antibody is suitable to detect IRX2 in samples from Human, Mouse, Rat, Dog, Horse, Guinea Pig and Cow. It has been validated for WB.

Quick Overview for IRX2 antibody (Middle Region) (ABIN2779496)

Target: IRX2 (Iroquois Homeobox Protein 2 (IRX2))

Reactivity: Human, Mouse, Rat, Dog, Horse, Guinea Pig, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRX2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IRX2 Antibody

Binding Specificity: Middle Region

Purpose: IRX2 Antibody - middle region

Sequence: LEDDEDDDEE GERGLAPPKP VTSSPLTGLE APLLSPPPEA APRGGRKTPQ

Predicted Reactivity: Cow: 79%, Dog: 93%, Guinea Pig: 91%, Horse: 93%, Human: 100%, Mouse: 92%, Rat: 83%

Characteristics: This is a rabbit polyclonal antibody against IRX2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human IRX2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8103479

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-IRX2 antibody (ABIN2779496)

Kadota, Sato, Duncan, Ooshima, Yang, Diaz-Meyer, Gere, Kageyama, Fukuoka, Nagata, Tsukada, Dunn, Wakefield, Lee: "Identification of novel gene amplifications in breast cancer and coexistence of gene amplification with an activating mutation of PIK3CA." in: Cancer research, Vol. 69, Issue 18, pp. 7357-65, (2009) (PubMed).

Target Details for IRX2

Target: IRX2 (Iroquois Homeobox Protein 2 (IRX2))

Alternative Name: IRX2

Background:

Background Information: IRX2 is a member of the Iroquois homeobox gene family. Members of this family appear to play multiple roles during pattern formation of vertebrate embryos.

Gene Name: Iroquois homeobox 2

Alternative Symbols: IRXA2

Protein Name: Iroquois-class homeodomain protein IRX-2

Molecular Weight: 49kDa

Gene ID: 153572

NCBI Accession: NP_150366

UniProt: Q9BZI1

Pathways: Tube Formation