POU1F1 antibody (Middle Region)

Catalog No. ABIN2779561

This Rabbit Polyclonal antibody specifically detects POU1F1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Goat, Horse, Rabbit, Sheep and Guinea Pig.

Quick Overview for POU1F1 antibody (Middle Region) (ABIN2779561)

Target: POU1F1 (POU Class 1 Homeobox 1 (POU1F1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Goat, Horse, Rabbit, Sheep, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU1F1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POU1F1 Antibody

Binding Specificity: Middle Region

Sequence: KRRTTISIAA KDALERHFGE QNKPSSQEIM RMAEELNLEK EVVRVWFCNR

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 93%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against POU1F1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human POU1F1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 291 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for POU1F1

Target: POU1F1 (POU Class 1 Homeobox 1 (POU1F1))

Alternative Name: POU1F1

Background: PIT1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals and is a member of the POU family of transcription factors that regulate mammalian development. The POU family is so named because the first 3 members identified were PIT1 and OCT1 of mammals, and Unc-86 of C. elegans. PIT1 contains 2 protein domains, termed POU-specific and POU-homeo, which are both necessary for high affinity DNA binding on genes encoding growth hormone and prolactin. PIT1 is also important for regulation of the genes encoding prolactin and thyroid-stimulating hormone beta subunit by thyrotropin-releasing hormone and cyclic AMP. This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: GHF-1, PIT1, Pit-1, Pit-1 beta, CPHD1, POU1F1a
Protein Interaction Partner: CEBPD, NCOR1, CREBBP, MED1, GATA2, NR1I3, NR1I2, PITX1, JUN, ETS1, NR3C1, VDR, PPARG, PPARA,
Protein Size: 291

Molecular Weight: 33 kDa

Gene ID: 5449

NCBI Accession: NM_000306, NP_000297

UniProt: P28069

Pathways: Regulation of Carbohydrate Metabolic Process